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KMID : 1145720220100020041
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Korean Herbal Medicine Informatics
2022 Volume.10 No. 2 p.41 ~ p.54
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Increased apoptosis of HT22 nerve cells of Cheonwangbosim-dan by Schisandra fruit
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No Kyoung-Ok
Noh Ga-Eun
Lee Gi-Hyun
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Abstract
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We studied the effect of Cheonwangbosim-dan (CWBSD) on oxidative stress-induced apoptosis in HT22 cells. We examined the effect of CWBSD on oxidative stress induced by H2O2, glutamate, or RSL3 in HT22 cells. In addition, 14 herbal medicines constituting CWBSD were also performed. The antioxidant activities of CWBSD were assessed by measuring free radical scavenging activities on ABTS and DPPH. Flow cytometry and fluorescence microscopy confirmed the increase in intracellular reactive oxygen species (ROS). As a result, There was no toxicity in HT22 cells in CWBSD (25, 50, 100 μg/ml), and antioxidant activity was confirmed in DPPH and ABTS assays. In the environment given oxidative stress (H2O2, glutamate, RSL3), treatment with CWBSD increased intracellular ROS of HT22 cells and increased LDH release, thereby reducing cell viability. Among the 14 herbal medicines of CWBSD, Schizandrae Fructus decreased the cell viability, and 13 herbal medicines except Schizandrae Fructus had no effect. Schizandrae Fructus is thought to be toxic to HT22 cells against oxidative stress.
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KEYWORD
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Cheonwangbosim-dan (CWBSD), Tian Wang Bu Xin Dan, apoptosis, HT22
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